RNA

Part:BBa_K1212000:Design

Designed by: Aura Ferreiro   Group: iGEM13_UC_Davis   (2013-09-16)

Theophylline Riboswitch (Clone 8.1*)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 42
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The last seven base pairs provide necessary spacing between the riboswitch and the start codon of the gene of interest.


Source

References

[1] S. A. Lynch and J. P. Gallivan, "A flow cytometry-based screen for synthetic riboswitches," Nucleic Acids Research, vol. 37, pp. 184-192, Jan 2009.